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About

Advanced Light Microscopy Shared Resource

Overview of Services

 

Fluorescence, transmission, confocal and two-photon microscopes and cameras available for use by researchers on a fee for use basis.  Facility provides training, maintenance, troubleshooting and operator-assisted imaging.

 

 

The Advanced Light Microscopy Shared Resource aids basic and physician researchers to image and publish high profile articles that:

  • Elucidate cell and molecular mechanisms of cancer, immunologic, infectious, metabolic, neurologic and vascular diseases
  • Evaluate therapeutic efficacy in cells and patient samples
  • Test new nanotechnologies in cell based assays
  • Quantitatively measure changes in tissue morphology and pathology

 

The Advanced Light Microscopy Shared Resource provides UNM researchers access to state-of-the art instrumentation for multiple fluorescence and transmitted light microscopy techniques.

  • Laser scanning microscopes capable of multi-spectral imaging systems enable simultaneous visualization and quantification fluorescent labels
  • Fluorescence Lifetime Imaging Microscopy (FLIM) produces an image based on the differences in the excited state decay rate from a fluorescent sample. Thus, FLIM is a fluorescence imaging technique where the contrast is based on the lifetime of individual fluorophores rather than their emission spectra intensity.
  • Spinning disk confocal and sensitive digital cameras allow quantitative assessment of cell numbers in tissue sections and imaging of dynamic processes in real time
  • Ratiometric imaging allows quantification of real time changes in calcium and other ion concentrations in response to cell signaling

 

Resource staff offer investigators.

  • Expert consultation on experiment design and specimen preparation
  • Training
  • Imaging for a fee
  • Ongoing assistance

Leadership

 

Diane Lidke, PhD | Faculty Director

Email: DLidke@salud.unm.edu

Phone: 505-272-8375

 

Michael Paffett, PhD | Research Facility Director

Email: MPaffett@salud.unm.edu

Phone: 505-272-1437

 

 

 

Location and hours of operation

Hours Location

Monday - Friday

9:00 a.m. to 5:00 p.m.

 

Cancer Research Facility

Rooms 212-226

Links and Resources

  1. Advanced Light Microscopy Shared Resource website